Local and systemic biochemical alterations induced by Bothrops atrox snake venom in mice

Research Report

OpenAccess

J Venom Res (2012), Vol 3, 28-34

Published online: 25 October 2012

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Carlos AT de Souza †, Anderson M Kayano ‡, Sulamita S Setúbal †, Adriana S Pontes †, Juliana L Furtado †, Fábio H Kwasniewski †, Kayena D Zaqueo †, Andreimar M Soares †, Rodrigo G Stábeli †‡ and Juliana P Zuliani †‡*

† Laboratório de Bioquímica e Biotecnologia/Cultivo Celular, Instituto de Pesquisas em Patologias Tropicais, IPEPATRO/FIOCRUZ Rondônia, Porto Velho-RO, Brazil

‡ Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, FIOCRUZ Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR, Porto Velho-RO, Brazil


*Correspondence to: Juliana Zuliani, Email: jzuliani@pq.cnpq.br, Tel: +55 69 32196010, Fax: +55 69 32196000


Received: 23 May 2012, Revised: 09 August 2012, Accepted: 16 August 2012


© Copyright The Authors


ABSTRACT

The local and systemic alterations induced by Bothrops atrox snake venom (BaV) injection in mice were studied. BaV induced superoxide production by migrated neutrophils, mast cell degranulation and phagocytosis by macrophages. Moreover it was observed hemorrhage in dorsum of mice after 2h of BaV injection and 3h after the venom injection in gastrocnemius muscle, there was myonecrosis which was assessed by the determination of serum and tissue CK; besides the release of urea, but not creatinine and uric acid, indicating kidney alterations. Yet, BaV induce the release of LDH and transaminases (ALT and AST), and thereby indicating tissue and liver abnormalities. In conclusion, the data indicate that BaV induces local and systemic important events.

KEYWORDS: Snake venom, Bothrops atrox, leukocytes, hemorrhage, myonecrosis, renal failure, liver abnormalities