Venom gland components of the ectoparasitoid wasp, Anisopteromalus calandrae

Research Article

OpenAccess
J Venom Res 
(2015), Vol 6, 19-37

Published online: 24 December 2015

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Lindsey C Perkin*, Kenlee S Friesen, Paul W Flinn and Brenda Oppert

US Department of Agriculture, Agricultural Research Service, Center for Grain and Animal Health Research, 1515 College Avenue, Manhattan KS 66502, USA


*Correspondence to: Lindsey Perkin, Email: lindsey.perkin@ars.usda.gov


Received:  09 September 2015; Revised: 10 December 2015; Accepted: 23 December 2015


© Copyright The Author(s). First Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.


ABSTRACT

The wasp Anisopteromalus calandrae is a small ectoparasitoid that attacks stored product pest beetle larvae that develop inside grain kernels, and is thus a potential insect control tool. The components of A. calandrae venom have not been studied, but venom from other organisms contain proteins with potential applications such as pest management tools and treatments for human diseases. We dissected female A. calandrae and collected venom and associated glands. Using high throughput sequencing, a venom gland transcriptome was assembled that contained 45,432 contigs, 25,726 of which had BLASTx hits. The majority of hits were to Nasonia vitripennis, an ectoparasitoid from the same taxonomic family, as well as other bees, wasps, and ants. Gene ontology grouped sequences into eleven molecular functions, among which binding and catalytic activity had the most representatives. In this study, we highlighted the most abundant sequences, and those that are likely the functional components of the venom. Specifically, we focused on genes encoding proteins potentially involved in host developmental arrest, disrupting the host immune system, host paralysis, and transcripts that support these functions. Our report is the first to characterize components of the A. calandrae venom gland that may be useful as control tools for insect pests and other applications.

KEYWORDS: Anisopteromalus calandrae, venom transcriptome, venom gland, ectoparasitoid, insect pests