The relaxant effect of the Montivipera bornmuelleri snake venom on vascular contractility

Research Report

OpenAccess
J Venom Res 
(2016), Vol 7, 10-15

Published online: 23 February 2016

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Claudine Accary ¹,*, Souad Hraoui-Bloquet 2, Riyad Sadek 3, Asma Alameddine 4, Ziad Fajloun 1,5, Jean-Claude Desfontis and Yassine Mallem 5

Laboratory of Applied Biotechnology (LAB3B), Azm Center for Research in Biotechnology and its Applications, Doctoral School of Science and Technology, Lebanese University, El Mittein Street, Tripoli, Lebanon

Faculty of Sciences II (Fanar), Lebanese University, B.P. 90656 Jdeidet El Maten, Lebanon

American University, Department of Marine Sciences, Beirut, Lebanon

BNMI, UMR CNRS 6214 – INSERM1083, University of Angers, France

Faculty of sciences III, Lebanese University, Tripoli, Lebanon

LUNAM, Oniris, Unit of Animal Pathophysiology and Functional Pharmacology, Nantes, France


*Correspondence to: Claudine Accary, Email: claudineaccary@hotmail.com, Tel: + 961 6 213 255, Fax: + 961 6 213 383


Received:  20 August 2015; Revised: 14 February 2016; Accepted: 14 February 2016


© Copyright The Author(s). First Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.


ABSTRACT

Molecular richness of snake venoms is an important source of proteins and toxins with potent effects on the cardiovascular system. The alteration of the vascular system in the victim after a venomous snake bite is usually expressed by a significant decrease in blood pressure. Therefore, exploring snake venom to extract and characterize its biomolecules is of considerable medical interest, and formed the basis of this study. We assessed the potential of the venom of Montivipera bornmuelleri, a viper from Lebanon, to induce relaxant effect on isolated Wistar rat aorta via several mechanisms of action. The overall hypotensive effect of Montivipera bornmuelleri venom results from its synergetic action on different channels for the reduction of blood pressure. By actions of its metalloproteinases and phospholipase A2, the venom may induce the production of nitric oxide acting accordingly a vasodilator effect. It could act on the voltage-dependent potassium channels and/or the L-type calcium channels, inhibiting angiotensin converting enzyme and/or inhibiting the α1-adrenoceptors. This work demonstrates vasorelaxant effect of the Montivipera bornmuelleri venom acting on different pathways, reducing blood pressure.

KEYWORDS: Montivipera bornmuelleri, snake venom, cardiovascular, blood pressure, aortic rings, vasorelaxant, calcium channels