Evidence for in vitro antiophidian properties of aqueous buds extract of Eucalyptus against Montivipera bornmuelleri venom

Research Report

OpenAccess
J Venom Res 
(2017), Vol 8, in press

Published online: 18 October 2017

Full Text (PDF ~230kb)

Joseph Khoury1,2 , Ranin Dabbousy1, Riyad Sadek3, Sayed Antoun4, Walid Hleihel5, Christian Legros2,* and Ziad Fajloun1,6,*

1LAB3B, Azm Centre for Research in Biotechnology and its Application, EDST, Lebanese University, Tripoli, Lebanon

2Mitochondrial and Cardiovascular Pathophysiology – MITOVASC, Team 2, Cardiovascular Mechanotransduction, UMR CNRS 6015, INSERM U1083, UBL/Angers University, Angers, France

3merican University of Beirut, Department of Marine Sciences, Beirut, Lebanon

4Faculty of Sciences III, Department of Chemistry and Biochemistry, Lebanese University, Tripoli, Lebanon

5Faculty of Sciences, USEK, Mount-Lebanon, Lebanon

6Faculty of Science III, Department of Biology, Lebanese University, Tripoli, Lebanon


*Correspondence to: Ziad Fajloun, E-mail: ziad.fajloun@ul.edu.lb; or Christian Legros, Email: christian.legros@univ-angers.fr


Received: 26 June 2017 | Revised: 07 October 2017 | Accepted: 18 October 2017


©Copyright The Author(s). First published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction of this article, provided the original work is appropriately acknowledged, with correct citation details.


ABSTRACT

Because snake venoms are complex mixtures of bioactive molecules, snake bites produce a large panel of symptoms which cannot be totally prevented by current antivenoms. Thus investigating plant extracts for antivenomics therapy approaches seemed relevant. Here, we evaluated the potency of the aqueous Buds extract of Eucalyptus (ABEE) to counteract the main enzymatic activities of Montivipera bornmuelleri venom. We showed that ABEE efficiently counteracts the proteolytic, Phospholipases A2 (PLA2), and L-aminoacid oxidase activities (LAAO) of M. bornmuelleri venom. ABEE was found to inhibit Acetylcholine esterase (AChE) and to exhibit a potent antioxidant activity. While In addition, M. bornmuelleri venom displays antibacterial properties against Staphylococcus aureus, which were not inhibited by ABEE. We also showed that of M. bornmuelleri venom lacks AChE, either anti-AChE activities. ABEE represents a promising natural source of antivenomics compounds against the deleterious effects of M. bornmuelleri or other Vipera species bites.

KEYWORDS: Montivipera bornmuelleri venom, Antivenomic approaches, Aqueous bud extract of Eucalyptus, Natural anti-venoms.