Notice: Function _load_textdomain_just_in_time was called incorrectly. Translation loading for the newsstand domain was triggered too early. This is usually an indicator for some code in the plugin or theme running too early. Translations should be loaded at the init action or later. Please see Debugging in WordPress for more information. (This message was added in version 6.7.0.) in /home/u636552293/domains/jvenomres.co.uk/public_html/wp-includes/functions.php on line 6170
Purification of a phospholipase A2 from Daboia russelii siamensis venom with anticancer effects – Journal of Venom Research
Saturday, July 18, 2026
Recent ArticlesResearch ArticlesVolume 2, 2011

Purification of a phospholipase A2 from Daboia russelii siamensis venom with anticancer effects

22 Oct 2011
0
Research Article

J Venom Res (2011), Vol 2, 42-51

Published online: 22 October 2011

Full Text: (PDF ~1767kb) | (PubMed Central Record HTML)

Suchitra Khunsap †‡, Narumol Pakmanee †, Orawan Khow †, Lawan Chanhome †, Visith Sitprija †, Montamas Suntravat ‡, Sara E Lucena ‡ , John C Perez ‡ , Elda E Sánchez ¥*

† Queen Saovabha Memorial Institute, Bangkok 10330, Thailand ‡ National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, MSC 158, 975 West Avenue B, Kingsville, TX 78363, USA

¥ Department of Chemistry and NNTRC, Texas A&M University-Kingsville, MSC 161, Kingsville, TX 78363, USA

*Correspondence to: Elda Sánchez, E-mail: elda.sanchez@tamuk.edu, Tel: +1 361 593 3796, Fax: +1 361 593 3798

Received: 08 September 2011, Revised: 15 October 2011, Accepted: 17 October 2011

© Copyright The Authors

ABSTRACT

Venom phospholipases A2 (PLA 2) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA 2 (Drs-PLA 2) was purified from Daboia russelii siamensisvenom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange high performance liquid chromatography (HPLC). The molecular weight of the Drs-PLA 2 was 13,679Da, which was determined by MALDI-TOF mass spectrometry. Its N-terminal amino acid sequence was homologous to basic PLA 2s of viperid snake venoms. The Drs-PLA 2 had indirect hemolytic and anticoagulant activities, cytotoxic activity with a CC 50 of 65.8nM, and inhibited SK-MEL-28 cell migration with an IC 50 of 25.6nM. In addition, the Drs-PLA 2 inhibited the colonization of B16F10 cells in lungs of BALB/c mice by ~65%.

KEYWORDS: Daboia russelii siamensis, p hospholipase A 2, cytotoxic, cell migration inhibition, lung tumor colonization